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Todas as publicações
de Biotecnologia
8147822
DY7Z65YY
1
apa
50
date
desc
12030
https://aclatam.cropscience.bayer.com/wp-content/plugins/zotpress/
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Slewinski, T. L., Turner-Hissong, S., Paciorek, T., Brower-Toland, B., & Shyu, C. (2025). Beautiful and delicious mutants: The origins, fates, and benefits of molecular sequence variation in plant evolution and breeding. Plant Physiology, kiaf378. https://doi.org/10.1093/plphys/kiaf378
Vertuan, H., Jose, M., Crivellari, A., G. Belchior, G., Verardino, L., J. Soares, D., Bellini, L. F., Bacalhau, F., Barancelli, M., Sordi, D., & Berger, G. (2023). Biomass degradation after harvest of genetically modified products compared to conventional counterparts. Journal of Regulatory Science, 11(1). https://doi.org/10.21423/JRS.REGSCI.111186
Barcellos, G. A., Hanich, M. R., Pretto, V. E., Weschenfelder, M. A. G., Horikoshi, R. J., Dourado, P. M., Ovejero, R. F. L., Berger, G. U., Martinelli, S., Head, G. P., & Bernardi, O. (2022). Characterizing the lethal and sub‐lethal effects of genetically modified soybean expressing Cry1A .105, Cry2Ab2 , and Cry1Ac insecticidal proteins against Spodoptera species (Lepidoptera: Noctuidae) in Brazil. Pest Management Science, ps.7225. https://doi.org/10.1002/ps.7225
Yu, W., Head, G. P., & Huang, F. (2022). Inheritance of Resistance to Cry1A.105 in Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae). Insects, 13(10), 875. https://doi.org/10.3390/insects13100875
Wang, C., Bean, G. J., Chen, C. J., Kessenich, C. R., Peng, J., Visconti, N. R., Milligan, J. S., Moore, R. G., Tan, J., Edrington, T. C., Li, B., Giddings, K. S., Bowen, D., Luo, J., Ciche, T., & Moar, W. J. (2022). Safety assessment of Mpp75Aa1.1, a new ETX_MTX2 protein from Brevibacillus laterosporus that controls western corn rootworm. PLOS ONE, 17(9), e0274204. https://doi.org/10.1371/journal.pone.0274204
Edrington, T., Wang, R., McKinnon, L., Kessenich, C., Hodge-Bell, K., Li, W., Tan, J., Brown, G., Wang, C., Li, B., & Giddings, K. (2022). Food and feed safety of the Bacillus thuringiensis derived protein Vpb4Da2, a novel protein for control of western corn rootworm. PLOS ONE, 17(8), e0272311. https://doi.org/10.1371/journal.pone.0272311
Lin, S., Head, G., Price, P., Niu, Y., & Huang, F. (2022). Relative fitness of susceptible and Cry1A.105/Cry2Ab2‐single‐/dual‐protein‐resistant Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) on non‐Bt diet and a diet containing a low concentration of two proteins. Insect Science, 1744-7917.13087. https://doi.org/10.1111/1744-7917.13087
Akkerdaas, J. H., Cianferoni, A., Islamovic, E., Kough, J., Ladics, G. S., McClain, S., Poulsen, L. K., Silvanovich, A., Pereira Mouriès, L., & van Ree, R. (2022). Impact of Food Matrices on Digestibility of Allergens and Poorly Allergenic Homologs. Frontiers in Allergy, 3, 909410. https://doi.org/10.3389/falgy.2022.909410
Carpane, P., & Catalano, M. I. (2022). Probing behavior of the corn leafhopper Dalbulus maidis on susceptible and resistant maize hybrids. PLOS ONE, 17(5), e0259481. https://doi.org/10.1371/journal.pone.0259481
Usovsky, M., Chen, P., Li, D., Wang, A., Shi, A., Zheng, C., Shakiba, E., Lee, D., Canella Vieira, C., Lee, Y., Wu, C., Cervantez, I., & Dong, D. (2022). Decades of Genetic Research on Soybean mosaic virus Resistance in Soybean. Viruses, 14(6), 1122. https://doi.org/10.3390/v14061122
Horikoshi, R. J., Ferrari, G., Dourado, P. M., Climaco, J. I., Vertuan, H. V., Evans, A., Pleau, M., Morrell, K., José, M. O. M. A., Anderson, H., Martinelli, S., Ovejero, R. F. L., Berger, G. U., & Head, G. (2022). MON 95379 Bt maize as a new tool to manage sugarcane borer ( Diatraea saccharalis ) in South America. Pest Management Science, ps.6986. https://doi.org/10.1002/ps.6986
Wang, C., Bean, G. J., Chen, C. J., Kessenich, C. R., Peng, J., Visconti, N. R., Milligan, J. S., Moore, R. G., Tan, J., Edrington, T. C., Li, B., Giddings, K. S., Bowen, D., Luo, J., Ciche, T., & Moar, W. J. (2022). Safety assessment of insecticidal proteins: A case study utilizing His-tagged protein to determine the safety of Mpp75Aa1.1, a new ETX_MTX2 protein that controls western corn rootworm [Preprint]. Pharmacology and Toxicology. https://doi.org/10.1101/2022.03.28.486136
Franz, L., Raming, K., & Nauen, R. (2022). Recombinant Expression of ABCC2 Variants Confirms the Importance of Mutations in Extracellular Loop 4 for Cry1F Resistance in Fall Armyworm. Toxins, 14(2), 157. https://doi.org/10.3390/toxins14020157
Zane, G. M., Silveria, M. A., Meyer, N. L., White, T. A., & Chapman, M. S. (2022). Cryo-EM Structure of Adeno-associated virus-4 at 2.2 Å resolution [Preprint]. Microbiology. https://doi.org/10.1101/2022.01.12.476110
Darlington, M., Reinders, J. D., Sethi, A., Lu, A. L., Ramaseshadri, P., Fischer, J. R., Boeckman, C. J., Petrick, J. S., Roper, J. M., Narva, K. E., & Vélez, A. M. (2022). RNAi for Western Corn Rootworm Management: Lessons Learned, Challenges, and Future Directions. Insects, 13(1), 57. https://doi.org/10.3390/insects13010057
Kouranov, A., Armstrong, C., Shrawat, A., Sidorov, V., Huesgen, S., Lemke, B., Boyle, T., Gasper, M., Lawrence, R., & Yang, S. (2022). Demonstration of targeted crossovers in hybrid maize using CRISPR technology. Communications Biology, 5(1), 53. https://doi.org/10.1038/s42003-022-03004-9
Gierse, R. M., Oerlemans, R., Reddem, E. R., Gawriljuk, V. O., Alhayek, A., Baitinger, D., Jakobi, H., Laber, B., Lange, G., Hirsch, A. K. H., & Groves, M. R. (2022). First crystal structures of 1-deoxy-d-xylulose 5-phosphate synthase (DXPS) from Mycobacterium tuberculosis indicate a distinct mechanism of intermediate stabilization. Scientific Reports, 12(1), 7221. https://doi.org/10.1038/s41598-022-11205-9
Glenn, K. C., Silvanovich, A., Lee, S. G., Allen, A., Park, S., Dunn, S. E., Kessenich, C., Meng, C., Vicini, J. L., & Jez, J. M. (2022). Biochemical and clinical studies of putative allergens to assess what distinguishes them from other non-allergenic proteins in the same family. Transgenic Research, 31(4–5), 507–524. https://doi.org/10.1007/s11248-022-00316-8
Horikoshi, R. J., Dourado, P. M., Bernardi, O., Willse, A., Godoy, W. A., Omoto, C., Bueno, A. de F., Martinelli, S., Berger, G. U., Head, G. P., & Corrêa, A. S. (2022). Regional pest suppression associated with adoption of Cry1Ac soybean benefits pest management in tropical agriculture. Pest Management Science, 78(10), 4166–4172. https://doi.org/10.1002/ps.7034
Groen, K., Trimbos, K. B., Hein, S., Blaauw, A. I., van Bodegom, P. M., Hahne, J., & Jacob, J. (2022). Establishment of a faecal DNA quantification technique for rare and cryptic diet constituents in small mammals. Molecular Ecology Resources, 22(6), 2220–2231. https://doi.org/10.1111/1755-0998.13609
Dai, S., Georgelis, N., Bedair, M., Hong, Y., Qi, Q., Larue, C. T., Sitoula, B., Huang, W., Krebel, B., Shepard, M., Su, W., Kretzmer, K., Dong, J., Slewinski, T., Berger, S., Ellis, C., Jerga, A., & Varagona, M. (2022). Ectopic expression of a rice triketone dioxygenase gene confers mesotrione tolerance in soybean. Pest Management Science, 78(7), 2816–2827. https://doi.org/10.1002/ps.6904
Bauman, P. A., Doxey, A. C., Eberini, I., Islamovic, E., Jungo, F., Kessenich, C., Kough, J., Krishan, M., Palazzolo, L., Privalle, L., Rodriguez, C. E., Satchell, K. J. F., Silvanovich, A., & Pereira Mouriès, L. (2022). “From Protein Toxins to Applied Toxicological Testing” virtual workshop identifies the need for a bioinformatic framework to assess novel food protein safety. Regulatory Toxicology and Pharmacology, 131, 105146. https://doi.org/10.1016/j.yrtph.2022.105146
Otim, M. H., Alibu, S., Asea, G., Abalo, G., Sserumaga, J. P., Adumo, S., Alupo, J., Ochen, S., Tefera, T., Bruce, A. Y., Beyene, Y., Meisel, B., Tende, R., Nang’ayo, F., Baguma, Y., Mugo, S., & Oikeh, S. O. (2022). Performance of Bt maize event MON810 in controlling maize stem borers Chilo partellus and Busseola fusca in Uganda. Crop Protection, 156, 105945. https://doi.org/10.1016/j.cropro.2022.105945
Sidorov, V., Wang, D., Nagy, E. D., Armstrong, C., Beach, S., Zhang, Y., Groat, J., Yang, S., Yang, P., & Gilbertson, L. (2022). Heritable DNA-free genome editing of canola (Brassica napus L.) using PEG-mediated transfection of isolated protoplasts. In Vitro Cellular & Developmental Biology - Plant, 58(3), 447–456. https://doi.org/10.1007/s11627-021-10236-7
Santiago González, J. C., Kerns, D. L., Head, G. P., & Yang, F. (2022). Status of Cry1Ac and Cry2Ab2 resistance in field populations of Helicoverpa zea in Texas, USA. Insect Science, 29(2), 487–495. https://doi.org/10.1111/1744-7917.12947
Bakker, F., Aldershof, S., Braaker, S., Dinter, A., Elston, C., Kroder, S., Mayer, C.-J., Pilling, E., & Neumann, P. (2022). Calibration of laboratory derived indices for non-target arthropod risk assessment with field data for plant protection products. Ecotoxicology and Environmental Safety, 235, 113427. https://doi.org/10.1016/j.ecoenv.2022.113427
Hernández‐Martínez, P., Bretsnyder, E. C., Baum, J. A., Haas, J. A., Head, G. P., Jerga, A., & Ferré, J. (2022). Comparison of in vitro and in vivo binding site competition of Bacillus thuringiensis Cry1 proteins in two important maize pests. Pest Management Science, 78(4), 1457–1466. https://doi.org/10.1002/ps.6763
Moar, W. J., Giddings, K. S., Narva, K. E., & Nelson, M. E. (2022). Enhancing global food security by using bacterial proteins with improved safety profiles to control insect pests. Journal of Invertebrate Pathology, 187, 107704. https://doi.org/10.1016/j.jip.2021.107704
Kuwar, S. S., Mishra, R., Banerjee, R., Milligan, J., Rydel, T., Du, Z., Xie, Z., Ivashuta, S., Kouadio, J.-L., Meyer, J. M., & Bonning, B. C. (2022). Engineering of Cry3Bb1 provides mechanistic insights toward countering western corn rootworm resistance. Current Research in Insect Science, 2, 100033. https://doi.org/10.1016/j.cris.2022.100033
Reinders, J. D., Wangila, D. S., Robinson, E. A., French, B. W., & Meinke, L. J. (2021). Characterizing the Relationship Between Western Corn Rootworm (Coleoptera: Chrysomelidae) Larval Survival on Cry3Bb1-Expressing Corn and Larval Development Metrics. Journal of Economic Entomology, 114(5), 2096–2107. https://doi.org/10.1093/jee/toab151
Yang, P., Havecker, E., Bauer, M., Diehl, C., Hendrix, B., Hoffer, P., Boyle, T., Bradley, J., Caruano-Yzermans, A., & Deikman, J. (2021). Beyond identity: Understanding the contribution of the 5’ nucleotide of the antisense strand to RNAi activity. PLOS ONE, 16(9), e0256863. https://doi.org/10.1371/journal.pone.0256863
Jenkins, D., Dobert, R., Atanassova, A., & Pavely, C. (2021). Impacts of the regulatory environment for gene editing on delivering beneficial products. In Vitro Cellular & Developmental Biology - Plant. https://doi.org/10.1007/s11627-021-10201-4
van Ree, R., Sapiter Ballerda, D., Berin, M. C., Beuf, L., Chang, A., Gadermaier, G., Guevera, P. A., Hoffmann-Sommergruber, K., Islamovic, E., Koski, L., Kough, J., Ladics, G. S., McClain, S., McKillop, K. A., Mitchell-Ryan, S., Narrod, C. A., Pereira Mouriès, L., Pettit, S., Poulsen, L. K., … Bowman, C. (2021). The COMPARE Database: A Public Resource for Allergen Identification, Adapted for Continuous Improvement. Frontiers in Allergy, 2, 700533. https://doi.org/10.3389/falgy.2021.700533
Towles, T. B., Buntin, G. D., Catchot, A. L., Gore, J., Cook, D. R., Caprio, M. A., & Daves, C. (2021). Quantifying the Contribution of Seed Blended Refugia in Field Corn to Helicoverpa zea (Lepidoptera: Noctuidae) Populations. Journal of Economic Entomology, 114(4), 1771–1778. https://doi.org/10.1093/jee/toab097
Martin, M., Boaventura, D., & Nauen, R. (2021). Evaluation of Reference Genes and Expression Level of Genes Potentially Involved in the Mode of Action of Cry1Ac and Cry1F in a Susceptible Reference Strain of Chrysodeixis includens. Insects, 12(7), 598. https://doi.org/10.3390/insects12070598
Chen, D., Moar, W. J., Jerga, A., Gowda, A., Milligan, J. S., Bretsynder, E. C., Rydel, T. J., Baum, J. A., Semeao, A., Fu, X., Guzov, V., Gabbert, K., Head, G. P., & Haas, J. A. (2021). Bacillus thuringiensis chimeric proteins Cry1A.2 and Cry1B.2 to control soybean lepidopteran pests: New domain combinations enhance insecticidal spectrum of activity and novel receptor contributions. PLOS ONE, 16(6), e0249150. https://doi.org/10.1371/journal.pone.0249150
Kessenich, C., & Silvanovich, A. (2021). Challenges of Automation and Scale: Bioinformatics and the Evaluation of Proteins to Support Genetically Modified Product Safety Assessments. Journal of Invertebrate Pathology, 107587. https://doi.org/10.1016/j.jip.2021.107587
Deslous, P., Bournonville, C., Decros, G., Okabe, Y., Mauxion, J.-P., Jorly, J., Gadin, S., Brès, C., Mori, K., Ferrand, C., Prigent, S., Ariizumi, T., Ezura, H., Hernould, M., Rothan, C., Pétriacq, P., Gibon, Y., & Baldet, P. (2021). Overproduction of ascorbic acid impairs pollen fertility in tomato. Journal of Experimental Botany, 72(8), 3091–3107. https://doi.org/10.1093/jxb/erab040
Kumar, S., Rymarquis, L. A., Ezura, H., & Nekrasov, V. (2021). Editorial: CRISPR-Cas in Agriculture: Opportunities and Challenges. Frontiers in Plant Science, 12. https://doi.org/10.3389/fpls.2021.672329
Ivashuta, S., Iandolino, A., & Watson, G. (2021). Exogenous RNA as a Regulatory Signal during a Plant’s Interaction with the Biotic Environment: An Evolutionary Perspective and Future Applications in Agriculture. Plants (Basel, Switzerland), 10(3). https://doi.org/10.3390/plants10030532
Willse, A., Flagel, L., & Head, G. (2021). Estimation of Cry3Bb1 resistance allele frequency in field populations of western corn rootworm using a genetic marker. G3 Genes|Genomes|Genetics, 11(1), jkaa013. https://doi.org/10.1093/g3journal/jkaa013
Yu, W., Lin, S., Dimase, M., Niu, Y., Brown, S., Head, G. P., Price, P. A., Reay-Jones, F. P. F., Cook, D., Reisig, D., Thrash, B., Ni, X., Paula-Moraes, S. V., & Huang, F. (2021). Extended investigation of field-evolved resistance of the corn earworm Helicoverpa zea(Lepidoptera: Noctuidae) to Bacillus thuringiensis Cry1A.105 and Cry2Ab2 proteins in thesoutheastern United States. Journal of Invertebrate Pathology, 107560. https://doi.org/10.1016/j.jip.2021.107560
Nauen, R., Zimmer, C. T., & Vontas, J. (2021). Heterologous expression of insect P450 enzymes that metabolize xenobiotics. Current Opinion in Insect Science, 43, 78–84. https://doi.org/10.1016/j.cois.2020.10.011
Mehlhorn, S., Hunnekuhl, V. S., Geibel, S., Nauen, R., & Bucher, G. (2021). Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide. Frontiers in Zoology, 18(1), 60. https://doi.org/10.1186/s12983-021-00444-7
Horikoshi, R. J., Dourado, P. M., Berger, G. U., de S. Fernandes, D., Omoto, C., Willse, A., Martinelli, S., Head, G. P., & Corrêa, A. S. (2021). Large-scale assessment of lepidopteran soybean pests and efficacy of Cry1Ac soybean in Brazil. Scientific Reports, 11(1), 15956. https://doi.org/10.1038/s41598-021-95483-9
Huang, L., Lümmen, P., & Berry, E. A. (2021). Crystallographic investigation of the ubiquinone binding site of respiratory Complex II and its inhibitors. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 1869(9), 140679. https://doi.org/10.1016/j.bbapap.2021.140679
Hendrix, B., Zheng, W., Bauer, M. J., Havecker, E. R., Mai, J. T., Hoffer, P. H., Sanders, R. A., Eads, B. D., Caruano-Yzermans, A., Taylor, D. N., Hresko, C., Oakes, J., Iandolino, A. B., Bennett, M. J., & Deikman, J. (2021). Topically delivered 22 nt siRNAs enhance RNAi silencing of endogenous genes in two species. Planta, 254(3), 60. https://doi.org/10.1007/s00425-021-03708-y
Dimase, M., Brown, S., Head, G. P., Price, P. A., Walker, W., Yu, W., & Huang, F. (2021). Performance of Bt‐susceptible and ‐heterozygous dual‐gene resistant genotypes of Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) in seed blends of non‐Bt and pyramided Bt maize. Insect Science, 28(4), 1147–1158. https://doi.org/10.1111/1744-7917.12850
Windus, L. C. E., Jones, A. M., Downes, S., Walsh, T., Knight, K., & Kinkema, M. (2021). HearNPV susceptibility in Helicoverpa armigera and Helicoverpa punctigera strains resistant to Bt toxins Cry1Ac, Cry2Ab, and Vip3Aa. Journal of Invertebrate Pathology, 183, 107598. https://doi.org/10.1016/j.jip.2021.107598
Purugganan, M. D., & Jackson, S. A. (2021). Advancing crop genomics from lab to field. Nature Genetics, 53(5), 595–601. https://doi.org/10.1038/s41588-021-00866-3
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Este comunicado pode conter declarações prospectivas baseadas em suposições e previsões atuais feitas pela administração da Bayer. Vários riscos conhecidos e desconhecidos, incertezas e outros fatores podem levar a diferenças materiais entre os resultados futuros reais, condição financeira, desenvolvimento ou desempenho da empresa e as estimativas fornecidas aqui. Esses fatores incluem aqueles discutidos nos relatórios públicos da Bayer que estão disponíveis no site da Bayer em www.bayer.com. A empresa não assume a responsabilidade de atualizar essas declarações prospectivas ou de adaptá-las a eventos ou desenvolvimentos futuros.